Hypoxia increases invasiveness of isolated term trophoblast cells.
Affiliation and adress for correspondence

Pracownia Badań nad Łożyskiem przy Katedrze i Zakładzie Patologii Ogólnej i Doświadczalnej, Akademia Medyczna w Warszawie.
Kierownik Katedry: prof. dr hab. n. med. S. Maśliński
Correspondence to: Pracownia Badań nad Łożyskiem, Katedra i Zakład Patologii Ogólnej i Doświadczalnej Akademii Medycznej w Warszawie, ul. Krakowskie Przedmieoecie 26/28, 00-927 Warszawa
Source of financing: Department own sources

GIN ONKOL, 2005, 3 (2), p. 98-105
ABSTRACT

Introduction: The aim of this study was to determine the influence of hypoxia on invasiveness of the human term trophoblast cultured in vitro. Material and method: Placental samples, obtained in standardized manner after normal term pregnancies (N=6) were subjected to enzymatic digestion, and the resultant cell suspension was filtrated and centrifuged in 5-70% Percoll. The cell layers between Percoll 40-50% were collected and developed into two trophoblast cultures: normoxic (20% O2; group I), and hypoxic (2% O2; group 2). In order to examine trophoblast invasiveness, the Cell Invasion Assay Kit (Chemicon International, USA), equipped with extra cellular matrix proteins-coated permeable polycarbonate membrane, was used. After 48 hours, invasive cells on the bottom surface of the polycarbonate membrane were counted in both groups in calibrated areas, using morphometric software. Results: The mean number of identified trophoblastic cells in the group II amounted 184.57±19%±SD of the mean number counted in the Group I. Conclusions: The statistically significant (p=0.05) increase of the trophoblast, cultured in vitro in hypoxic conditions, when compared with the culture in normoxic conditions, was ascertained. The trophoblast cells in full-term pregnancy preserve the ability to answer for local hypoxia, typical for the first trimester trophoblast.

Keywords: hypoxia, trophoblast culture, invasiveness, human placenta